Translational_Unit

Part:BBa_K322139:Design

Designed by: Richard Partridge-Hicks and Maria Kowal   Group: iGEM10_Edinburgh   (2010-09-25)

Blue emitting luciferase (from X. luminescens)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


Design Notes

We prepared this for two reasons: firstly to resolve an issue with the SpeI site in our submitted version of K216008, and to put it in pSB1C3, since this seems to be the new standard vector for the Registry.

Source

Genomic DNA of Xenorhabdus (Photorhabdus) luminescens Hb

References